INFLUENCE OF CULTIVATION TEMPERATURE AND YERSINIA PESTIS FRA-OPERON CARRIAGE ON MORPHOLOGICAL FEATURES OF YERSINIA PSEUDOTUBERCULOSIS


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Abstract

Aim. Studies of influence of Yersinia pestis fra-operon carriage on morphological properties of Y.pseudotuberculosis recipient strain cells and colonies at different temperature and cultivation time. Materials and methods. Cultures of Y.pseudotuberculosis isogenic variants were grown on solid nutrient medium at 4—6, 9—11, 26—28 and 36—38°C. Indirect hemagglutination was used to determine F1 antigen production. Cytorefractometry was used to determine live cell percentage in colonies. Size and dividing cells percentage was evaluated by using phase-contrast microscope «Lyumam-I2». Results. Time period between appearance of microcolonies and achievement of maximum colony size increased with cultivation temperature decrease. Size of fra+ and fra- variant colony was not significantly different for every temperature regiment used and was significantly lower at the temperature of 36—38°C. Conclusion. Temperature has a significant influence on population growth, colony size and form.

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ВЛИЯНИЕ ТЕМПЕРАТУРЫ КУЛЬТИВИРОВАНИЯ И НОСИТЕЛЬСТВА FRA-ОПЕРОНА YERSINIA PESTIS НА МОРФОЛОГИЧЕСКИЕ ОСОБЕННОСТИ YERSINIA PSEUDOTUBERCULOSIS
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About the authors

L. B Gavrilova

48 Central Research Institute of Russian Defense Ministry, Kirov, Russia

A. A Byvalov

Research Institute of Physiology, Syktyvkar, Russia

A. V Chernyadev

48 Central Research Institute of Russian Defense Ministry, Kirov, Russia

K. E Gavrilov

48 Central Research Institute of Russian Defense Ministry, Kirov, Russia

S. L Kuznetsov

48 Central Research Institute of Russian Defense Ministry, Kirov, Russia

I. V Borysevich

48 Central Research Institute of Russian Defense Ministry, Kirov, Russia

References

  1. Бывалов А.А., Гаврилов К.Е., Крупин В.В. и др. Биологические и физико-химические свойства культур бактерий Yersinia pseudotuberculosis, несущих fra-оперон Yersinia pestis. Мол. генет. микробиол. вирусол. 2008, 1: 14-18.
  2. Варвашевич Т.Н., Сидорова В.Е. Вопросы микробиологии, патогенеза и лабораторной диагностики иерсиниозов. Новосибирск, 1985.
  3. Дармов И.В., Маракулин И.В., Янов С.Н. и др. Конструирование штаммов-продуцентов антигенов F1 и T чумного микроба. Биотехнология. 1992, 6: 59-62.
  4. Печенкин Д.В., Бывалов А.А., Маракулин И.В. и др. Влияние капсулы Yersinia pestis на фагоцитарную активность перитонеальных макрофагов морской свинки. Пробл. особо опасн. инфекц. 2005, 90(2): 47-48.
  5. Сомов Г.П., Покровский В.И., Беседнова Н.Н. и др. Псевдотуберкулез. М., 2001.
  6. Фихман Б.А. Микробиологическая рефрактометрия. М., 1967.

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Copyright (c) 2011 Gavrilova L.B., Byvalov A.A., Chernyadev A.V., Gavrilov K.E., Kuznetsov S.L., Borysevich I.V.

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