CHARACTERISTIC OF BIOLOGICAL PROPERTIES OF NEISSERIA GONORRHOEAE AND PARAMETERS OF LOCAL IMMUNITY DURING UROGENITAL GONOCOCCAL INFECTION


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Abstract

Aim. To study the prevalence and expression of antilactoferrin, IgAand sIgA-protease activity of gonococci and state of local immunity during various forms of urogenital gonorrhea. Materials and methods. Ability to inactivate lactoferrin (ALfA), IgA and secretory IgA (sIgA) was studied in 28 Neisseria gonorrhoeae strains isolated from patients with localized gonorrhea and 26 strains isolated from patients with systemic signs of gonorrhea. State of the local immunity was assessed on the lactoferrin, IgA and sIgA levels, which were measured by immunofluorescence assay in ejaculate of 54 patients with gonorrhea and 18 healthy males. Results. Penetrance of ALfA, IgAand sIgA-protease activity of gonococci did not depend from form of infection. Expression of studied characteristics of gonococci as well as combination of ALfA and sIgA-protease activities were more prominent in patients with systemic signs of gonorrhea. The same patients had higher level of lactoferrin in semen and, in contrast, lower levels of IgA and sIgA compared with patients with localized gonorrhea. Conclusion. Strains of gonococci inactivating lactoferrin, IgA and sIgA depress mucosal barrier of urogenital biotope and create conditions for the development of disseminated forms of gonococcal infection.

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ХАРАКТЕРИСТИКА БИОЛОГИЧЕСКИХ СВОЙСТВ NEISSERIA GONORRHOEAE И ПАРАМЕТРОВ МЕСТНОГО ИММУНИТЕТА ПРИ УРОГЕНИТАЛЬНОЙ ГОНОКОККОВОЙ ИНФЕКЦИИ
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About the authors

O. O Mikhaylova

Orenburg State Medical Academy, Orenburg, Russia

I. N Chaynikova

Orenburg State Medical Academy, Orenburg, Russia

E. A Mikhaylova

Orenburg State Medical Academy, Orenburg, Russia

A. I Smolyagin

Orenburg State Medical Academy, Orenburg, Russia

References

  1. Ахунова Н.Р. Антикомплементарная активность гонококков. Автореф.дис. канд.мед.наук. Оренбург, 1999.
  2. Бухарин О.В., Валышев А.В., Чайникова И.Н. и др. Способ определения антилактоферриновой активности микроорганизмов. Патент на изобретение 2245923. Бюл. 4 от 12.03.03.
  3. Бухарин О.В., Чайникова И.Н., Валышев А.В. и др. Способ определения антииммуноглобулиновой активности микроорганизмов. Патент на изобретение 2236465. Бюл. 26 от 20.09.04.
  4. Валышев А.В., Валышева И.В. Роль антилактоферриновой активности бактерий в их персистенции. Журн. микробиол. 2006, 4: 23-25.
  5. Воронина Л.Г. Совершенствование диагностики, прогнозирования и лечения гонореи под контролем антилизоцимного признака гонококков. Автореф. дис. д-ра мед. наук. Оренбург, 1998.
  6. Канышкова Т.Г., Бунева В.Н., Невинский Г.А. Лактоферрин и его биологические функции. Биохимия. 2001, 66 (1): 5-13.
  7. Beck S.C., Meyer T.F. IgAl protease from Neisseria gonorrhoeae inhibits TNFalpha-mediated apoptosis of human monocytic cells. FEBS Lett. 2000, 28 (2—3): 287-292.
  8. Hopper S., Vasqucs В., Merz A. et al. Effeci of the immunoglobulin Al protease on Neisseria genorrhoeae trafficking across polarized T84 epithelial monolayers. Infect. Immun. 2000, 68: 906-911.
  9. Kilian M., Reinholt J., Poulsen K. et al. Biological significance of IgAl protease in bacterial colonization and pathogenesis: critical evaluation of experimental evidence. APMIS. 1996, 104: 321-338
  10. Lorenzen D.R., Dux F., Wolk U. et al. Immunoglobulin Al protease, an exoenzyme of pathogenic Neisseria, is a potent inducer of proinflammatory cytokines. J. Exp. Med. 1999, 190: 1049-1058.
  11. Rao M.B., Тanksale A.M., Ghatge M.S. et al. Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. Rev. 1998, 62: 597-635.
  12. Ulvatne Н., Haukland Н.Н., Samuelsen О. et al. Proteases in Escherichia coli and Staphylococcus aureus confer reduced susceptibility to lactoferricin В. J. Antimicrob. Chemother. 2002, 50: 461-467.
  13. Vitovski S.R., Read C., Sayers J.R. Invasive isolated of Neisseria meningitidis possess enhanced immunoglobulin AI protease activity compared to colonizing strains. FASEB J. 1999, 13: 331-337.

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Copyright (c) 2009 Mikhaylova O.O., Chaynikova I.N., Mikhaylova E.A., Smolyagin A.I.

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