STUDY OF EFFECT OF THERMOLABILE ENTEROTOXIN OF ENTEROBACTER CLOACAE ON EXPRESSION OF CYTOKINE GENES IN MICE

Aim . To study the role of production of several cytokines by macrophages and neutrophils in experiment. Materials and methods . Production of cytokines — IL-2, 4, 6, 10, 12, 15 as well as interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), transforming growth factor β (TGF-β) by macrophages and neutrophils during experiment on mice inoculated with enterotoxigenic strain of Enterobacter cloacae was measured. Expression of cytokines was determined by reverse transcription PCR and dot-hybridization on the basis of specially synthesized nucleotide sequences. Results. In macrophages of experimental animals inoculated with enterotoxigenic strain of E.cloacae increased production of TGF-β, IFN-γ and decreased level of TNF-α were revealed. Transcription of mRNAs of IL-15 and IL-6 was detected in macrophages and neutrophils of experimental animals at 24 h after inoculation. Transcription of mRNAs of IL-2, IL-4 and IL-10 was detected neither in macrophages nor in neutrophils. Conclusion . Thermolabile enterotoxin of E.cloacae renders negative effect on IFN-γ, promotes enhanced expression of TGF-β and suppresses production of IL-2, IL-4, IL-10. Absence of expression of key cytokines suggests that effect of thermolabile enterotoxin of Enterobacter bacteria excludes it from cytokine regulation of immune response during infectious process.

interleukins, thermolabile enterotoxin, tumor necrosis factor α, transforming growth factor β, macrophages, neutrophils, experiment