CONTEMPORARY MOLECULAR-GENETIC METHODS USED FOR ETIOLOGIC DIAGNOSTICS OF SEPSIS

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Abstract

Etiologic diagnostics of sepsis is one of the most difficult problems of contemporary medicine due to a wide variety of sepsis causative agents, many of which are components of normal human microflora. Disadvantages of contemporary «golden standard» of microbiologic diagnostics of sepsis etiology by seeding of blood for sterility are duration of cultivation, limitation in detection of non-cultivable forms of microorganisms, significant effect of preliminary empiric antibiotics therapy on results of the analysis. Methods of molecular diagnostics that are being actively developed and integrated during the last decade are deprived of these disadvantages. Main contemporary methods of molecular-biological diagnostics are examined in the review, actual data on their diagnostic characteristic are provided. Special attention is given to methods of PCR-diagnostics, including novel Russian developments. Methods of nucleic acid hybridization and proteomic analysis are examined in comparative aspect. Evaluation of application and perspectives of development of methods of molecular diagnostics of sepsis is given.

About the authors

S. N. Gavrilov

Central Research Institute of Epidemiology

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

T. S. Skachkova

Central Research Institute of Epidemiology

Email: noemail@neicon.ru
Russian Federation

O. Yu. Shipulina

Central Research Institute of Epidemiology

Email: noemail@neicon.ru
Russian Federation

Yu. A. Savochkina

Central Research Institute of Epidemiology

Email: noemail@neicon.ru
Russian Federation

G. A. Shipulin

Central Research Institute of Epidemiology

Email: noemail@neicon.ru
Russian Federation

V. V. Maleev

Central Research Institute of Epidemiology

Email: noemail@neicon.ru
Russian Federation

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Copyright (c) 2016 Gavrilov S.N., Skachkova T.S., Shipulina O.Y., Savochkina Y.A., Shipulin G.A., Maleev V.V.

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