Email this article REGULATION OF MICROORGANISMS SENSITIVITY TO ANTAGONISTIC ACTIVE REPRESENTATIVES OF MUTUALISTICALLY MICROFLORA BY THE ANTIMICROBIAL PEPTIDES
- Authors: Sycheva M.V.1,2, Peshkova Y.I.2, Kartashova O.L.1,2, Andreeva A.V.3
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Affiliations:
- institute of Cellular and Intracellular Symbiosis
- Orenburg State Agrarian University
- Bashkir State Agrarian University
- Issue: Vol 94, No 6 (2017)
- Pages: 21-25
- Section: Articles
- URL: https://microbiol.crie.ru/jour/article/view/225
- DOI: https://doi.org/10.36233/0372-9311-2017-6-21-25
- ID: 225
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Abstract
Aim. Study of the effect of antimicrobial peptides (AMP) on the sensitivity of the conditional pathogenic of bacteria to the action of antagonistic active of representatives mutualistically microflora. Materials and methods. The study used peptides from platelets of chicken obtained by the method of reversed-phase high-performance liquid chromatography in stepwise and linear gradients of increasing concentrations of organic solvent. The effect of AMP on the sensitivity of microorganisms to antagonistic active substances of the dominant bacteria of the genus Lactobacillus, and Enterococcus were investigated in a joint incubation. Antagonistic activity of bacteria studied the gup method. Results. Shown an increased expression of antagonistic activity of indigenous microflora in relation to associative microflora after concubinage with a peptide fractions from platelets of chicken. Conclusion. The obtained results allow to assume the existence of new mechanisms of formation of the colonization resistance of the biotope.
About the authors
M. V. Sycheva
institute of Cellular and Intracellular Symbiosis; Orenburg State Agrarian University
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
YU. I. Peshkova
Orenburg State Agrarian University
Email: noemail@neicon.ru
Russian Federation
O. L. Kartashova
institute of Cellular and Intracellular Symbiosis; Orenburg State Agrarian University
Email: noemail@neicon.ru
Russian Federation
A. V. Andreeva
Bashkir State Agrarian University
Email: noemail@neicon.ru
Russian Federation
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