Molecular genetic characteristics of Vibrio cholerae nonO1/nonO139 strains isolated on the territory of Russian Federation from patients with otitis
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| 1. | Title | Title of document | Molecular genetic characteristics of Vibrio cholerae nonO1/nonO139 strains isolated on the territory of Russian Federation from patients with otitis |
| 2. | Creator | Author's name, affiliation, country | Elena V. Monakhova; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 2. | Creator | Author's name, affiliation, country | Alexey S. Vodop'yanov; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 2. | Creator | Author's name, affiliation, country | Vladimir D. Kruglikov; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 2. | Creator | Author's name, affiliation, country | Nadezhda A. Selyanskaya; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 2. | Creator | Author's name, affiliation, country | Ruslan V. Pisanov; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 2. | Creator | Author's name, affiliation, country | Alexey K. Noskov; Rostov-on-Don Plague Control Researsh Institute; Россия |
| 3. | Subject | Discipline(s) | |
| 3. | Subject | Keyword(s) | Vibrio cholerae nonO1/nonO139; extraintestinal forms of NAG infections; whole genome sequences; bioinformatics analysis; pathogenicity/persistence factors |
| 4. | Description | Abstract | Introduction. In 2017–2020 for the first time in many years strains of Vibrio cholerae nonO1/nonO139 (NAGs) were isolated in Russia from patients with otitis. Aim — bioinformatic analysis of whole genome sequences (WGSs) and sequences of individual genes of NAG strains - causative agents of otitis isolated in Russia. Materials and methods. Analysis of WGSs of eight NAG clinical isolates obtained on the MiSeq Illumina platform was carried out using BioEdit, BLASTN, BLASTP, Vector NTI programs; antibiotic resistance was determined according to MUK 4.2.2495-09. Results. The strains differed in SNP content, sets of determinants of pathogenicity/persistence factors and their alleles. All lacked CTX, preCTX, RS1 prophages, VPI pathogenicity island, thermostable toxin gene, mobile elements associated with antibiotic resistance, pandemicity island VSP-I; two strains contained VSP-II island. Genes of a number of proteases, cholix toxin, type 3 secretion system (T3SS) cluster and additional T6SS clusters formed different combinations. Products of the altered genes retained or lost their characteristic active domains. In the cytotoxin MARTX of 6 strains, the key ACD domain was absent; in 4 strains a new rtxA-like domain was revealed. Biofilm gene clusters varied in their structure. The presence of genes for antibiotic resistance did not always correlate with antibioticograms. All strains were susceptible to most antibiotics, but some showed resistance to 1–4 drugs. Conclusion. All the studied strains — causative agents of otitis, in spite of revealed differences, have sufficient sets of determinants responsible for realization of pathogenic and persistent potential. Due to discrepancy between the genotypic and phenotypic characteristics of antibiotic resistance, one should rely mainly on the phenotype when choosing drugs for the etiotropic therapy of NAG infections. Emergence of patients with otitis caused by NAG-vibrios in Russia indicates the advisability of the inclusion of tests for their identification in the scheme of bacteriological analysis for extraintestinal infections and, in cases of their isolation, for prompt determination of sensitivity to antibiotics. |
| 5. | Publisher | Organizing agency, location | Central Research Institute for Epidemiology |
| 6. | Contributor | Sponsor(s) | |
| 7. | Date | (DD-MM-YYYY) | 27.09.2022 |
| 8. | Type | Status & genre | Peer-reviewed Article |
| 8. | Type | Type | Research Article |
| 9. | Format | File format | |
| 10. | Identifier | Uniform Resource Identifier | https://microbiol.crie.ru/jour/article/view/1315 |
| 10. | Identifier | Digital Object Identifier (DOI) | 10.36233/0372-9311-215 |
| 11. | Source | Title; vol., no. (year) | Journal of microbiology, epidemiology and immunobiology; Vol 99, No 4 (2022) |
| 12. | Language | English=en | ru |
| 13. | Relation | Supp. Files |
Table 1. V. cholerae nonO1/nonO139 clinical strains used in this study (61KB) Table 2. Reference sequences used as prototypes in comparative analysis (31KB) Table 3. Genes used for identification of antibiotic resistance determinants in WGSs of NAGs (134KB) Fig. 1. Phylogenetic tree based on cluster analysis (UPGMA) of distribution of 3683 SNPs located in open reading frames. Only SNPs that were not associated with other mutations within flanking 9 nucleotydes were included in analysis. (33KB) Fig. 2. Phylogenetic tree based on the results of AlignX analysis of MARTX of NAG vibrios (a) and topography of domains in their molecules (b). (261KB) Table 4. Minimal inhibitory concentrations (mg/l) for V. cholerae nonO1/nonO139 strains and presence of genes associated with antibiotic resistance (58KB) |
| 14. | Coverage | Geo-spatial location, chronological period, research sample (gender, age, etc.) | |
| 15. | Rights | Copyright and permissions |
Copyright (c) 2022 Monakhova E.V., Vodop'yanov A.S., Kruglikov V.D., Selyanskaya N.A., Pisanov R.V., Noskov A.K. This work is licensed under a Creative Commons Attribution 4.0 International License. |