Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology94210.36233/0372-9311-2020-97-6-4UnknownEpidemiology and molecular genetic characteristics of Lyme borreliosis pathogens circulating in tick’s population in the Almaty oblast of the Republic of KazakhstanBissenbayA. O.<p>Akerke O. Bissenbay — researcher, Department of biosafety and biosecurity</p><p>050054, Almaty</p>akerke.bissenbay@gmail.comhttps://orcid.org/0000-0002-7109-2534ZhigailovA. V.<p>Andrey V. Zhigailov — PhD (Biol.), leading researcher, Laboratory of molecular biology</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0002-9646-033XPerfilyevaYu. V.<p>Yuliya V. Perfilyeva — PhD (Biol.), leading researcher, Laboratory for expertise and diagnostics</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0001-6803-0773NaizabayevaD. A.<p>Dinara A. Naizabayeva — junior researcher, Laboratory for expertise and diagnostics</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0002-0606-4289NeupokoyevaA. S.<p>Alena S. Neupokoyeva — senior researcher, Laboratory for expertise and diagnostics</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0001-7257-8037BerdygulovaZh. A.<p>Zhanna A. Berdygulova — PhD, leading researcher, Laboratory of molecular biology</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0003-0379-2472OstapchukYe. O.<p>Yekaterina O. Ostapchuk — PhD, leading researcher, Laboratory of molecular biology</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0002-3771-423XMaltsevaE. R.<p>Elina R. Maltseva — PhD, Head, Biosafety and biosecurity department</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0001-9198-695XKuatbekovaS. A.<p>Saltanat A. Kuatbekova — junior researcher, Laboratory for expertise and diagnostics</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0001-5569-1847NizkorodovaA. S.<p>Anna S. Nizkorodova — PhD (Biol.), leading researcher, Laboratory of molecular biology</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0002-1597-7207DmitrovskiyA. M.<p>Andrey M. Dmitrovskiy — D. Sci. (Med.), Prof., Head, Laboratory for expertise and diagnostics</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0003-4714-3079SkibaYu. A.<p>Yuriy A. Skiba — PhD (Biol.), Deputy director</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0003-4895-1473MamadaliyevS. M.<p>Seydigapbar M. Mamadaliyev — D. Sci. (Vet.), Prof., Director</p><p>050054, Almaty</p>fake@neicon.ruhttps://orcid.org/0000-0002-7767-0251Almaty Branch of Republican State Enterprise «National Center for Biotechnology» — Central Reference Laboratory200120219765355452001202120012021Copyright © 2021, Bissenbay A.O., Zhigailov A.V., Perfilyeva Y.V., Naizabayeva D.A., Neupokoyeva A.S., Berdygulova Z.A., Ostapchuk Y.O., Maltseva E.R., Kuatbekova S.A., Nizkorodova A.S., Dmitrovskiy A.M., Skiba Y.A., Mamadaliyev S.M.2021<p><strong>Background</strong>. Information on the geographical distribution of different species of the <em>Borrelia burgdorferi</em> sensu lato (<em>B. burgdorferi</em> s.l.) complex is of great epidemiological importance, since different genospecies are associated with certain clinical manifestations of Lyme borreliosis. Although Almaty region of the Republic of Kazakhstan is considered to be endemic for tick-borne borreliosis, there is still no accurate data on the level of borrelia infection in ticks in the region, including information on the genotypes of circulating borrelia.</p>
<p><strong>The aim</strong> of this work was to study ticks collected from humans in the Almaty region of the Republic of Kazakhstan in 2018.</p>
<p><strong>Materials and methods</strong>. Ticks were tested for the presence of <em>B. burgdorferi</em> s.l. DNA, genotyping of the identified borrelia was done by sequencing of the fragment of 16S rRNA gene. The analysis of epidemiological data on the incidence of Lyme borreliosis in the Almaty region in 20132018 was performed.</p>
<p><strong>Results</strong>. <em>Rhipicephalus turanicus</em> (116/253), <em>Haemaphysalis punctata</em> (74/253), <em>Dermacentor marginatus</em> (28/253), and <em>Ixodes persulcatus</em> (23/253) were the predominant species of ticks taken from humans. The prevalence of <em>B. burgdorferi</em> s.l. infection in <em>I. persulcatus</em> ticks was 39.13% (9/23) It should be noted that the DNA of <em>B. burgdorferi</em> s.l. was also detected in single individuals of <em>D. marginatus</em>, <em>H. punctata</em>, and <em>R. turanicus</em>, although these species are not considered as competent <em>B. burgdorferi</em> s.l. vectors.</p>
<p><strong>Conclusion</strong>. As a result of sequencing of the positive homogenates of ticks, two genotypes of B. burgdorferi s.l. were identified: <em>B. afzelii</em> and <em>B. garinii</em> and/or <em>B. bavariensis</em>. Thus, at least two genospecies, <em>B. afzelii</em> and <em>B. garinii</em> and/or <em>B. bavariensis</em>, circulate in the territory of the Almaty region.</p>Lyme borreliosisBorrelia burgdorferi s.l.genospecies16S rRNA gene16S sequencingЛайм-боррелиозBorrelia burgdorferi s.l.геновидыген 16S рРНК16S секвенирование[Halperin J.J. Lyme Disease: an Evidence-Based Approach. Wallingford; 2018. https://doi.org/10.1079/9781786392077.0000][Margos G., Vollmer S.A., Ogden N.H., Fish D. Population genetics, taxonomy, phylogeny and evolution of Borrelia burgdorferi sensu lato. Infect. Genet. Evol. 2011; 11(7): 1545–63. https://doi.org/10.1016/j.meegid.2011.07.022][Adam T., Gossman G.S., Rasiah C., Göbel U.B. 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