Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology5210.36233/0372-9311-2016-3-70-74COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFICATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REALTIME PCR)PanferovaYu. A.noemail@neicon.ruFreilikhmanO. A.noemail@neicon.ruTokarevichN. K.noemail@neicon.ruKarpenkoS. F.noemail@neicon.ruGalimzyanovKh. M.noemail@neicon.ruPasteur Research Institute of Epidemiology and MicrobiologyAstrakhan State Medical University28062016933707410042019Copyright © 2016, Panferova Y.A., Freilikhman O.A., Tokarevich N.K., Karpenko S.F., Galimzyanov K.M.2016Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). Conclusion. This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process.Coxiella burnetiiQ feverCoxiella burnetiiPCRreal-time PCRsensitivitydiagnostic effectivenessлихорадка КуПЦРПЦР в режиме реального временичувствительностьдиагностическая эффективность[Бармин А.Н., Колчин Е.А., Шуваев Н.С., Дмитриева М.В. Анализ проявления природно-очаговых заболеваний на территории Астраханской обл. Естес. науки. 2012, 3 (40): 44-50.][Садретдинов Р.А, Галимзянов Х.М. Гемодинамические типы микроциркуляции у больных инфекционными лихорадками. Фундамент, исслед. 2010, 7: 63-66.][Фрейлихман О.А., Панфёрова Ю.А., Токаревич Н.К. Совершенствование метода детекции Coxiella burnetii в биологическом материале на основе амплификации гена groEL. Журн. микробиол. 2010, 4: 71-74.][Arricau-Bouvery N., HauckY., BejaouiA. etal. Molecular characterisation of Coxiella burnetii isolates by infrequent restriction site-PC Rand MLVA typing. BMC Microbiology. 2006,6 (38). doi: 10.1186/1471-2180-6-38.][Bielawska-Drozd A., Cieslik P., Mirski T. et al. Q fever - selected issues. Ann. Agric. Environmental Med. 2013, 20 (2): 222-232.][Freylikhman O., Tokarevich N., Suvorov A. et al. persistence in three generation of mice after application of live attenuated human M-44 vaccine against Q fever. Ann. N.Y. Acad. Sci. 2003, 990: 496-499.][Jaton K., Peter O., Raoult D. et al. Development of a high throughput PCR to detect Coxiella burnetii and its application in a diagnostic laboratory over a 7-year period. New Microbes New Infect. 2013, 1 (1): 6-12. doi: 10/1002/2052-2975.8.][MarrieT., Raoult D. Qfever-a review and issues for the next century. Int. J. Antimicrob. Agents. 1997, 8 (3): 145-161. doi: 10.1016/S0924-8579(96)00369-X.][Maurin M., Raoult D. Q fever. Clin. Microbiol. Rev. 1999, 12 (4): 518-553.][Raele D., Garofolo G., Galante D. et al. Molecular detection of Coxiella burnetii using an alternative loop-mediated isothermal amplification assay. Veterinaria Italiana. 2015, 51 (1): 73-78. doi: 10.12834/VetIt.3041168.4.][Tilburg J., Melchers W., Pettersson A. et al. Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii in serum. J. Clin. Microbiol. 2010, 48 (11): 3924-3927. doi: 10.1128/JCM.01006-10.][van Schaik E., Chen C., Mertens K. et al. Molecular pathogenesis of obligate intracellular bacterium. Nat. Rev. Microbiol. 2013, 11 (8): 561-573. doi: 10.1038/nrmicro3049.]