Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology51910.36233/0372-9311-2020-97-1-26-31Review ArticleThe Microbiota Continuum along the Reproductive Tract in Women with InfertilityGodovalovA. P.<p><strong>Anatoly P. Godovalov</strong> — Cand. Sci. (Med.), Assoc. Prof., Department of microbiology with a course of virology</p><p><em>Perm 614990</em><em></em></p>fake@neicon.ruKarpuninaT. I.<p><strong>Tamara I. Karpunina</strong> — Doct. Sci. (Biol.), Prof., Department of microbiology with a course of virology</p><p><em>Perm 614990</em><em></em></p>fake@neicon.ruE.A. Vagner Perm State Medical University0204202097126310503202005032020Copyright © 2020, Godovalov A.P., Karpunina T.I.2020<p><strong>Introduction.</strong> At present, the question of commensal, including opportunistic, microflora participation in infertility development remains debatable. In a number of studies, the translocation of the vaginal microflora into the endometrial tissue is considered as a factor contributing to inflammation development. In addition, the connection of some reproductive losses with the persistence of certain conditionally pathogenic microorganisms is shown. Today, to solve this issue, molecular genetic research methods are being actively introduced that surpass the routine cultivation techniques in a number of positions.</p>
<p>The <strong>aim</strong> of the study was to assess the taxonomic diversity of microorganisms in the vaginal biotope with infertility.</p>
<p><strong>Material and methods.</strong> For the study samples of vaginal contents obtained from the posterior vaginal fornix of 15 women, consisting of barren marriage, were used. A metagenomic study of 16S ribosomal RNA samples was carried out on the Illumina MiSeq platform, using the MiSeq Reagent Kits v3 kit (600-Cycle Kit), as recommended by the manufacturer. Libraries for sequencing plots of the V3-V4 gene of the 16S ribosomal RNA were prepared according to the 16S Metagenomic Sequencing Library Preparation Illumina. In bioinformatics assessment, Kraken Metagenomics version 2.0.0 software for metagenomic analysis (classifier of reads short nucleotide sequences) was used using a standard database.</p>
<p><strong>Results.</strong> It was shown that the occurrence of representatives of the <em>Lactobacillaceae</em> family in the vaginal biotope varies from 12 to 84%. The genus <em>Lactobacillus</em> with the dominance of <em>L. jensenii</em>, <em>L. delbrueckii</em> and <em>L. amylolyticus</em> occupied the leading position among the members of the family. In all samples, <em>Moraxella</em> spp. was found in large numbers, with <em>M. osloensis</em> leading among the representatives of the entire community. In half of the cases, the joint presence of <em>M. osloensis</em> and <em>G. vaginalis</em> was revealed.</p>
<p><strong>Conclusion.</strong> It has been established that in infertility in the vaginal microbiota the number of representatives of the genus <em>Lactobacillus</em> is significantly reduced, and there is also a change in the leading species to <em>L. jensenii</em>, whose functional activity does not fully ensure the colonization resistance of the vaginal biotope, allowing for excessive reproduction of oppotrunistic microorganisms, in particular, <em>M. osloensis</em>.</p>vaginal microbiotametagenomic sequencingL. jenseniiM. osloensisinfertilityвагинальная микробиотаметагеномное секвенированиеL. jenseniiM. osloe nsisбесплодие[Chen C., Song X., Wei W., Zhong H., Dai J., Lan Z., et al. The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases. Nat. Commun. 2017; 8(1): 875. DOI: http://doi.org/10.1038/s41467-017-00901-0][Stone L. Infection: Vaginal microbiota and infectious infertility. Nat. Rev. Urol. 2018; 15(3): 136. 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