Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology41210.36233/0372-9311-2018-3-40-44PECULIARITIES OF RECOMBINATIVE GENOMICS OF ACINETOBACTER — HUMAN PATHOGENSolomennyiA. P.Permfake@neicon.ruZubarevaN. A.Permfake@neicon.ruInstitute of Ecology and Genetics of MicroorganismsWagner State Medical University2507201895340442408201924082019Copyright © 2018, Solomennyi A.P., Zubareva N.A.2018<p><strong>Aim</strong>. The disclosure of the role of genetic markers variability among Acinetobacter genus in connection with multidrug-resistant phenotype realization. <strong>Materials and methods.</strong> A comparative analysis was reviewed on DNA fragments important for genetic recombination in A. baumannii — one of the most relevant pathogens of postoperative infection, as well as A. pittii and A. lwoffii. <strong>Results</strong>. Integrase/recombinase XerC gene-bearing region of the chromosome is notably different and could include the genes responsible for the development of resistance against polymyxins and (fluoro)quinolones, as well as other antibiotics. <strong>Conclusion</strong>. The results obtained are important in surveillance of epidemic (pandemic) strains of Acinetobacter spp.</p>Acinetobacter baumanniiepidemic (pandemic) cloneantibiotic resistancemultidrug
efflux transportersintegrase/recombinase XerCAcinetobacter baumanniiэпидемический (пандемический) клонантибиотикорезистентностьэффлюкс-транспортерыинтеграза/рекомбиназа XerC[1. Гончаров А.Е., Еремеева М.И., Зубарева Н.А., Соломенный А.П. Генотипический анализ карбапенем-устойчивого штамма Acinetobacter baumannii. Перм. мед. журнал. 2011, 28 (6): 95-99.][2. Сухорукова М.В., Эйдельштейн М.В., Склеенова Е.Ю., Иванчик Н.В., Тимохова А.В., Шек Е.А., Дехнич А.В., Козлов Р.С. и исследовательская группа «Марафон». Антибиотикорезистентность нозокомиальных штаммов Acinetobacter spp. в стационарах России: результаты многоцентрового эпидемиологического исследования МАРАФОН в 2011—2012 гг. Клин. микробиол. антимикроб. химиотер. 2014, 16 (4): 266-272.][3. Сухорукова М.В., Эйдельштейн М.В., Склеенова Е.Ю., Иванчик Н.В., Шек Е.А., Дехнич А.В., Козлов Р.С. и исследовательская группа «Марафон» Антибиотикорезистентность нозокомиальных штаммов Acinetobacter spp. в стационарах России: результаты многоцентрового эпидемиологического исследования «Марафон» 2013—2014. Клин. микробиол. антимикроб. химиотер. 2017, 19 (1): 42-48.][4. Чеботарь И.В., Лазарева А.В., Масалов Я.К., Михайлович В.М., Маянский Н.А. Acinetobacter: микробиологические, патогенетические и резистентные свойства. Вестник РАМН. 2014, 9-10: 39-50.][5. Antunes L.C., Visca P., Towner K.J. Acinetobacter baumannii: evolution of a global pathogen. Pathog. Dis. 2014, 71: 292-301.][6. Castillo F., Benmohamed A., Szatmari G. Xer site specific recombination: double and single recombinase systems. Front. Microbiol. 2017, 8: Art. 453.][7. Dettori M., Piana A., Deriu M.G. et al. Outbreak of multidrug-resistant Acinetobacter baumannii in an intensive care unit. New Microbiol. 2014, 37: 185-191.][8. Ellington M.J., Ekelund O., Aarestrup F.M. et al. The role of whole genome sequencing in antimicrobial susceptibility testing of bacteria: report from the EUCAST Subcommittee. Clin. Microbiol. Infect. 2017, 23: 2-22.][9. Harding C.M., Hennon S.W., Feldman M.F. Uncovering the mechanisms of Acinetobacter baumannii virulence. Nat. Rev. Microbiol. 2018. 16: 91-102.][10. Krzyciak P., Chmielarczyk A., Pobiega M. et al. Acinetobacter baumannii isolated from hospital-acquired infection: biofilm production and drug susceptibility. APMIS. 2017, 125: 1017-1026.][11. Olaitan A.O., Morand S., Rolain J-M. Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteria. Front. Microbiol. 2014, 5: Art. 643.][12. Oikonomou O., Sarrou S., Papagiannitsis C.C. et al. Rapid dissemination of colistin and carbapenem resistant Acinetobacter baumannii in Central Greece: mechanisms of resistance, molecular identification and epidemiological data. BMC Infect. Dis. 2015, 15: Art. 559.][13. Segagni Lusignani L., Starzengruber P., Dosch V. et al. Molecular epidemiology of multidrugresistant clinical isolates of Acinetobacter baumannii: A 10-year analysis in a large tertiary care university hospital in central Europe with international admissions. Wien Klin. Wochenschr. 2017, 129: 816-822.][14. Shafaati M., Boroumand M., Nowroozi J. et al. Correlation between qacE and qacEΔ1 efflux pump genes, antibiotic and disinfectant resistant among clinical isolates of E. coli. Recent Pat. Antiinfect. Drug Discov. 2016, 11: 189-195.][15. Wilharm G., Skiebe E., Higgins P.G. et al. Relatedness of wildlife and livestock avian isolates of the nosocomial pathogen Acinetobacter baumannii to lineages spread in hospitals worldwide. Environ. Microbiol. 2017, 19: 4349-4364.]