Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology31610.36233/0372-9311-2018-5-104-107THE PROBLEM OF CELL CULTURES CONTAMINATION WITH MAMMALIAN ORTHOREOVIRUSESFaisuloevE. B.noemail@neicon.ruKorchevayaE. P.noemail@neicon.ruMarkovD. V.noemail@neicon.ruPetrushaO. A.noemail@neicon.ruZverevV. V.noemail@neicon.ruMechnikov Research Institute of Vaccines and Sera2810201895510410710042019Copyright © 2018, Faisuloev E.B., Korchevaya E.P., Markov D.V., Petrusha O.A., Zverev V.V.2018A mandatory requirement for cell cultures used in scientific researches and biomanufacturing is the absence of their contamination by viruses. We have described the case of adventitious isolation of mammalian orthoreovirus in the rhesus macaque embryo kidney cells MA-104. PCR analysis for the presence of reovirus RNA of all probable sources of reovirus (trypsin, fetal bovine serum, clinical samples, cell culture) revealed no viral RNA in any of the samples. An important condition for the activation of the reovirus reproduction in the MA-104 cells was the presence of trypsin in the culture medium. The obtained results underscore the urgency of control for the reovirus contamination of chemicals of animal origin and cell cultures. Since reoviruses are associated with human diseases, such control in pharmaceutical production is mandatory.animal cell cultureviral contaminationmammalian orthoreovirustrypsinкультура животных клетоквирусная контаминацияортореовирус млекопитающихтрипсин[Колпаков С.А., Колпакова Е.П. Новая группа ротавирусов человека семейства Reoviridae. Электронное периодическое издание ЮФУ «Живые и биокосные системы». 2014, 10.][Правила проведения исследований биологических лекарственных средств Евразийского экономического союза. Утверждены Решением Совета Евразийской экономической комиссии от 3 ноября 2016 года № 89.][Dermody T.S. et al. Chapter 44. Orthoreoviruses. In: Fields Virology. David M. Knipe, Peter M. Howley. (ed.). 2013.][Golden J.W., Linke J., Schmechel S. et al. Addition of exogenous protease facilitates reovirus infection in many restrictive cells. J. Virol. 2002, 76(15): 7430-7443.][Hawkes P.W. Fetal bovine serum: geographic origin and regulatory relevance of viral contamination. Bioresour. Bioprocess. 2015, 2: 34.][Lelli D., Beato M.S., Cavicchio L. et al. First identification of mammalian orthoreovirus type 3 in diarrheic pigs in Europe. Virol. J. 2016, 13: 139.][Ouattara L.A., Barin F., Barthez M.A. et al. Novel human reovirus isolated from children with acute necrotizing encephalopathy. Emerg. Infect. Dis. 2011, 17(8): 1436-1444.][Sabin A.B. Reoviruses. A new group of respiratory and enteric viruses formerly classified as ECHO type 10 is described. Science. 1959, 130(3386): 1387-1389.][Steyer A., Gutierrez-Aguire I., Kolenc M. et al. High similarity of novel orthoreovirus detected in a child hospitalized with acute gastroenteritis to mammalian orthoreoviruses found in bats in Europe. J. Clin. Microbiol. 2013, 51(11): 3818-3825.][Thimmasandra Narayanappa A., Sooryanarain H., Deventhiran J. et al. A novel pathogenic Mammalian orthoreovirus from diarrheic pigs and Swine blood meal in the United States. MBio. 2015, 6(3): e00593-00515.]