Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology20210.36233/0372-9311-2017-5-53-58MICROBIOCENOSIS OF SKIN IN BROMHIDROSIS PATIENTSAleshkinA. V.noemail@neicon.ruBorisovaO. Yu.noemail@neicon.ruGaduaN. T.noemail@neicon.ruBochkarevaS. S.noemail@neicon.ruChernovaV. A.noemail@neicon.ruTrebunskikhI. P.noemail@neicon.ruEfimovB. A.noemail@neicon.ruKafarskayaL. I.noemail@neicon.ruAfanasievS. S.noemail@neicon.ruAleshkinV. A.noemail@neicon.ruAfanasievM. S.noemail@neicon.ruBorisovaA. B.noemail@neicon.ruKaraulovA. V.noemail@neicon.ruGabrichevsky Moscow Research Institute of Epidemiology and MicrobiologyGabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Pirogov Russian National Research Medical UniversityNational Medical Surgery Centre «Pirogov Children Consultant Diagnostic Centre»Centre of Hygiene and Epidemiology in MoscowPirogov Russian National Research Medical UniversitySechenov First Moscow State Medical University28102017945535810042019Copyright © 2017, Aleshkin A.V., Borisova O.Y., Gadua N.T., Bochkareva S.S., Chernova V.A., Trebunskikh I.P., Efimov B.A., Kafarskaya L.I., Afanasiev S.S., Aleshkin V.A., Afanasiev M.S., Borisova A.B., Karaulov A.V.2017Aim. Evaluate the composition of microorganisms of skin microbiocenosis of axilla in brom-hidrosis patients. Materials and methods. 23 patients were examined (11 - 17 years) under the observation at Pirogov CCDC of the National Medical-Surgery Centre. Identification was carried out using biochemical test-systems BioMerieux VITEK MS MALDI-TOF («bioMerieux», France) and 16SrRNA genesequencing with consequent juxtaposition with EMBL/NCBI. Medium and high degree of skin seeding with microbiota was present in most of the patients with bromhidrosis (52.2 and 43.5%). 137 strains belonging to 5 genera of microorganisms were identified - Corynebacterium, Staphylococcus, Moraxella, Micrococcus, Candida and Bacillus spp. Coiynehacte-rium genus strains (8 species) and Staphylococcus genus (5 species) prevailed in microbiocenosis (89.1%). C. tuberculostearicum strains dominated among Corynebacterium, and S. hominis - Staphylococcus. Conclusion. In most of the cases (82.6%) in patients microbiocenosis of skin of axilla was presented by consortiums of microorganisms with prevalence of Corynebacterium and Staphylococcus microorganisms.skin microbiotabromhidrosismicroorganism seedingaxillaмикробиота кожибромгидрозобсемененность микрорганимамиподмышечные впадины[Alatoom A., Cazanave C.J., Cunningham S.C. et al. Identification of non-diphtheriae Corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. JCM. 2013, 23: 160-163.][Bernard K. The genus Corvnebacterium and other medicallv relevant corvneform-like bacteria. JCM. 2012, 50 (10): 3152-3158.][Callewaert C., Kerckhof F.M., Granitsiotis M.S. et al. Characterization of Staphylococcus and Corynebacterium clusters in the human axillary region. Plos. 2013, 8 (8): e70538.][Chen Y.E., Tsao H. The skin microbiome: current perspectives and future challenges. J. Amer. Acad.Dermatology. 2013, 69 (1): 143-155.][Cogen A. L., Nizet V, Gallo R.L. Skin microbiota: a source of disease or defence? Br. J. Dermatol. 2008, 158 (3): 442-455.][Hinic V, Lang C, Weisser M. et al. Corynebacterium tuberculostearicum: a potentially misidentified and multiresistant Corynebacterium species isolated from clinical specimens. JCM. 2012,50 (8): 2561-2567.][Khamis A., Raoult D., Scola B. Comparison between rpoB and 16SrRNAgene sequencing for molecular identification of 168 clinical isolates of Corvnebacterium. J. Clinical Microbiology. 2005, 43 (4): 1934-1936.][Mathieu A., Delmont T.O., Vogel T.M. et al. Life on human surfaces: skin metagenomics. Plos. 2013, 8(6): e65288.][Mathieu A., Vogel T.M., Simonet P. The future of skin metagenomics. Res. Microbiol. 2014, 167:69-76.][Schommer N.N., Gallo R.L. Structure and function of the human skin microbiome. Trend Microbiol. 2013, 21 (12): 660-668.]