Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-7613Central Research Institute for Epidemiology1874610.36233/0372-9311-637KTUYXCORIGINAL RESEARCHESОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯResearch ArticleInsight from the genome sequence of Bacillus cereus BUKA harboring bla2 geneОсобенности последовательности генома Bacillus cereus BUKA, содержащего ген bla2https://orcid.org/0000-0001-6903-6392DavidEbuka
Nigeria

PhD, senior lecturer, Department of biochemistry

PhD, старший преподаватель каф. биохимии

david.ebuka@funai.edu.nghttps://orcid.org/0000-0002-2056-751XIgwenyiIkechukwu
Nigeria

PhD, Professor, Department of biochemistry

PhD, профессор каф. биохимии

igwenyike@yahoo.comhttps://orcid.org/0000-0002-8198-3955IrohaIfeanyichukwu
Nigeria

PhD, Professor, Department of microbiology

PhD, профессор каф. микробиологии

iriroha@yahoo.comhttps://orcid.org/0000-0001-7022-8922EzeiloUchenna
Nigeria

PhD, senior lecturer, Department of biochemistry

PhD, старший преподаватель каф. биохимии

uchenna.ezeilo@funai.edu.nghttps://orcid.org/0000-0001-9955-2785OgbanshiMoses
Nigeria

PhD, Associate Professor, Department of biochemistry

PhD, доцент каф. биохимии

ogbanshi.moses@funai.edu.nghttps://orcid.org/0000-0003-4612-196XObasiDavid
Nigeria

PhD, senior lecturer, Head, Department of biochemistry

PhD, старший преподаватель, зав. каф. биохимии

obasidc@dufuhs.edu.nghttps://orcid.org/0000-0001-8224-9579EjejeJerius
Nigeria

PhD, lecturer, Department of biochemistry

PhD, преподаватель каф. биохимии

ejeje.jerius@funai.edu.nghttps://orcid.org/0009-0009-8438-610XOgunwaShedrack
Nigeria

PhD, lecturer, Department of biochemistry

PhD, преподаватель каф. биохимии

ogunwa.shedrack@funai.edu.nghttps://orcid.org/0009-0007-0296-7134OnyemucheTochukwu
Nigeria

PhD, lecturer, Department of biochemistry

PhD, преподаватель каф. биохимии

tochukwu.onyemuche@funai.edu.nghttps://orcid.org/0000-0001-7927-8432KanuShedrach
Nigeria

PhD, lecturer, Department of biochemistry

PhD, преподаватель каф. биохимии

kanu.shedrack@funai.edu.nghttps://orcid.org/0000-0002-2945-4925EzennayaChidinma
Nigeria

lecturer, Department of biochemistry

преподаватель каф. биохимии

felicia.ezennaya@funai.edu.nghttps://orcid.org/0000-0002-1074-1553AlugbuoStanley
Nigeria

lecturer, Department of biochemistry

преподаватель каф. биохимии

alugbuo.udochukwu@funai.edu.nghttps://orcid.org/0009-0002-6200-2004IgbokeSunday
Nigeria

lecturer, Department of biochemistry

преподаватель каф. биохимии

igboke.sunday@funai.edu.nghttps://orcid.org/0000-0002-2550-4614DavidChidinma
Brazil

PhD Fellow, Escola Paulista de Medicina

аспирант Медицинской школы Паулиста

nnenne.nnaji@gmail.comhttps://orcid.org/0000-0001-9249-4922da SilvaAline
Brazil

PhD, Professor, Department of biochemistry, Institute of Chemistry, Escola Paulista de Medicina

PhD, профессор каф. биохимии Института химии, Медицинская школа Паулиста

almsilva@iq.usp.brAlex Ekwueme Federal University Ndufu Alike IkwoФедеральный университет имени Алекса Эквуэме Ндуфу Алике ИквоEbonyi State UniversityГосударственный университет ЭбониDavid Umahi Federal University of Health SciencesФедеральный университет медицинских наук им. Дэвид УмахиUniversidade Federal de São PauloФедеральный университет Сан-Паулу130320261031798523012025Copyright ©; 2026, David E., Igwenyi I., Iroha I., Ezeilo U., Ogbanshi M., Obasi D., Ejeje J., Ogunwa S., Onyemuche T., Kanu S., Ezennaya C., Alugbuo S., Igboke S., David C., da Silva A.Copyright ©; 2026, David E., Igwenyi I., Iroha I., Ezeilo U., Ogbanshi M., Obasi D., Ejeje J., Ogunwa S., Onyemuche T., Kanu S., Ezennaya C., Alugbuo S., Igboke S., David C., da Silva A.2026David E., Igwenyi I., Iroha I., Ezeilo U., Ogbanshi M., Obasi D., Ejeje J., Ogunwa S., Onyemuche T., Kanu S., Ezennaya C., Alugbuo S., Igboke S., David C., da Silva A.David E., Igwenyi I., Iroha I., Ezeilo U., Ogbanshi M., Obasi D., Ejeje J., Ogunwa S., Onyemuche T., Kanu S., Ezennaya C., Alugbuo S., Igboke S., David C., da Silva A.https://creativecommons.org/licenses/by/4.0https://microbiol.crie.ru/jour/article/view/18746

Introduction. In developing countries, yearly outbreaks of acute gastroenteritis records high rates of morbidity and mortality in children below five years.

Aim: present the whole genome sequence of Bacillus cereus BUKA implicated in acute childhood gastroenteritis.

Materials and methods. The sample was isolated from diarrheal stool of a child diagnosed with acute childhood gastroenteritis from a tertiary hospital in Nigeria.

Results. Antibiotics resistance profiling of the isolate revealed resistance to 79% (11/14) of the tested antibiotics including imipenem, penicillin, cefepime, ceftriaxone which suggests a strong ability to produce extended spectrum beta-lactamases, carbapenemases and cephalosporinases. Whole genome sequence insight confirmed the presence of chromosomally-encoded bla2 gene encoding enzyme that hydrolyze carbapenems, penicillin and cephalosporins. B. cereus encoding bla2 gene may be an emerging pathogen in the yearly incidence of acute childhood gastroenteritis in Nigeria.

Discussion. We herein report and implicate B. cereus harboring bla2 gene capable of hydrolyzing cephalosporins, and carbapenems as an emerging threat in acute childhood gastroenteritis in Nigeria

Введение. В развивающихся странах ежегодные вспышки острого гастроэнтерита приводят к высоким показателям заболеваемости и смертности среди детей в возрасте до 5 лет.

Цель — представить полную последовательность генома Bacillus cereus BUKA, связанного с острым гастроэнтеритом у детей.

Материалы и методы. Образец был выделен из жидкого стула ребёнка с диагнозом «острый детский гастроэнтерит» из больницы третьего уровня в Нигерии.

Результаты. Анализ антибиотикорезистентности изолята выявил резистентность к 79% (11/14) испытанных антибиотиков, включая имипенем, пенициллин, цефепим, цефтриаксон, что свидетельствует о высокой способности продуцировать бета-лактамазы расширенного спектра действия, карбапенемазы и цефалоспориназы. Анализ полной последовательности генома подтвердил наличие хромосомно-кодируемого гена bla2, кодирующего фермент, гидролизующий карбапенемы, пенициллин и цефалоспорины. B. cereus, кодирующий ген bla2, может быть новым патогеном в ежегодной заболеваемости острым детским гастроэнтеритом в Нигерии.

Обсуждение. В статье описывается наличие и влияние B. cereus, несущего ген bla2, способный гидролизовать цефалоспорины и карбапенемы, как новая угроза при остром гастроэнтерите у детей в Нигерии.

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