Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-7613Central Research Institute for Epidemiology1853810.36233/0372-9311-500mwauhyREVIEWSОБЗОРЫReview ArticleSubgingival microbiome in periodontal disease and comorbid pathology (meta-analysis)Поддесневой микробиом при заболеваниях пародонта и коморбидной патологии (метаанализ)https://orcid.org/0000-0001-9571-0520TsarevaTatyana V.ЦареваТатьяна Викторовна
Russian Federation

Cand. Sci. (Med.), Associate Professor, Department of microbiology, virology, immunology

к.м.н., доцент кафедры микробиологии, вирусологии, иммунологии

nikola777@rambler.ruhttps://orcid.org/0000-0001-8194-2419BalmasovaIrina P.БалмасоваИрина Петровна
Russian Federation

D. Sci. (Med.), Professor, Head, Laboratory of pathogenesis and methods of treatment of infectious diseases, Scientific Research Medical Dental Institute

д.м.н., профессор, зав. лаб. патогенеза и методов лечения инфекционных заболеваний Научно-исследовательского медицинского стоматологического института

nikola777@rambler.ruhttps://orcid.org/0000-0002-3311-0367TsarevVictor N.ЦаревВиктор Николаевич
Russian Federation

D. Sci. (Med.), Professor, Head, Department of microbiology, virology, immunology

д.м.н., профессор, зав. каф. микробиологии, вирусологии, иммунологии

nikola777@rambler.ruRussian University of MedicineРоссийский университет медицины10052024101228129210022024Copyright ©; 2024, Tsareva T.V., Balmasova I.P., Tsarev V.N.Copyright ©; 2024, Царева Т.В., Балмасова И.П., Царев В.Н.2024Tsareva T.V., Balmasova I.P., Tsarev V.N.Царева Т.В., Балмасова И.П., Царев В.Н.https://creativecommons.org/licenses/by/4.0https://microbiol.crie.ru/jour/article/view/18538

The problem of chronic periodontitis (CP) is actively discussed due to the recognition of the fact that periodontal microbial damage is closely related to a number of systemic diseases and probably plays an important role in the occurrence of comorbid pathology.

The aim of the meta-analysis was to characterize the composition of the subgingival microbiome and to determine the peculiarities of the formation of associations of the new periodontal pathogen Filifactor alocis with other I and II order periodontal pathogenic bacteria, as well as with the commensal bacteria colonizing this biotope.

The study presents data of patient examination with obligatory use of polymerase chain reaction methods and sequencing of 16S rRNA genes in 1529 healthy individuals and 2394 patients with CP, 136 patients with CP and concomitant atherosclerosis, 258 patients with CP and concomitant type 2 diabetes mellitus. It was confirmed that the basis of the oral microbiome under normal circumstances is composed of representatives of microaerophilic streptococci, corynebacteria, lactobacilli, as well as representatives of the Veillonella and Sphingobacterium genera. 16S sequencing and bioinformatic analysis allowed us to specify the taxonomic place of the new pathogen F. alocis, as well as representatives of normal microbiota in CP and comorbid somatic pathology.

Проблема хронического пародонтита (ХП) активно обсуждается в связи с признанием того факта, что микробное поражение пародонта тесно связано с рядом системных заболеваний и, вероятно, играет важную роль в возникновении коморбидной патологии.

Цель метаанализа — характеристика состава поддесневого микробиома и определение особенностей формирования ассоциаций нового пародонтопатогена Filifactor alocis с другими пародонтопатогенными бактериями I и II порядка, а также комменсальными бактериями, колонизирующими данный биотоп.

В исследовании представлены данные обследования пациентов с обязательным использованием методов полимеразной цепной реакции и секвенированием генов 16S рРНК у 1529 здоровых лиц и 2394 пациентов с ХП, 136 человек с ассоциацией ХП и атеросклероза, 258 человек с ассоциацией ХП и сахарного диабета 2-го типа. Подтверждено, что основу орального микробиома в норме составляют представители микроаэрофильных стрептококков, коринебактерий, лактобацилл, а также представителей родов Veillonella и Sphingobacterium. Проведённое 16S-секвенирование и биоинформационный анализ позволили конкретизировать таксономическое место нового возбудителя F. alocis, а также представителей нормобиоты при ХП и коморбидной соматической патологии.

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