Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-7613Central Research Institute for Epidemiology1847810.36233/0372-9311-470zphlvmREVIEWSОБЗОРЫReview ArticleRecombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseasesРекомбиназная полимеразная амплификация: характеристика метода и применение в диагностике инфекционных заболеванийhttps://orcid.org/0000-0001-5690-6686BondarevaOlga S.БондареваОльга Сергеевна
Russian Federation

Cand. Sci. (Med.), senior researcher, Laboratory of gene diagnostics of particularly dangerous infections

к.м.н., с.н.с. лаб. генодиагностики особо опасных инфекций

bondareva0s@mail.ruhttps://orcid.org/0000-0001-9510-7246BaturinArtem A.БатуринАртем Александрович
Russian Federation

researcher, Laboratory of gene diagnostics of particularly dangerous infections

н.с. лаб. генодиагностики особо опасных инфекций

bondareva0s@mail.ruhttps://orcid.org/0000-0002-6958-7861MironovaAnna V.МироноваАнна Владимировна
Russian Federation

researcher, Laboratory of gene diagnostics of particularly dangerous infections

н.с. лаб. генодиагностики особо опасных инфекций

bondareva0s@mail.ruVolgograd Plague Control Research InstituteВолгоградский научно-исследовательский противочумный институт10052024101227028021112023Copyright ©; 2024, Bondareva O.S., Baturin A.A., Mironova A.V.Copyright ©; 2024, Бондарева О.С., Батурин А.А., Миронова А.В.2024Bondareva O.S., Baturin A.A., Mironova A.V.Бондарева О.С., Батурин А.А., Миронова А.В.https://creativecommons.org/licenses/by/4.0https://microbiol.crie.ru/jour/article/view/18478

Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA). This review contains information about the principle of RPA, the role of individual reaction components and primer design considerations. It provides information on characteristics of various methods of RPA results detection, effects of inhibitors, temperature and agitation on the efficiency of reaction. Approaches to quantitative and multiplex RPA are described, as well as some variants of portable devices designed to identify infectious agents. The conclusion summarizes advantages and disadvantages of RPA in comparison with other amplification methods.

В последние годы технологии на основе изотермической амплификации активно развиваются и постепенно внедряются в арсенал методов диагностики инфекционных заболеваний. Одним из наиболее быстрых изотермических методов является рекомбиназная полимеразная амплификация (РПА). Данный обзор содержит информацию о принципе РПА, значении отдельных компонентов реакции и характеристике праймеров. Включены сведения об особенностях различных способов детекции результатов РПА, влиянии ингибиторов, температуры и перемешивания на эффективность реакции. Описаны подходы к проведению количественной и мультиплексной РПА, а также некоторые варианты портативных устройств для выявления возбудителей инфекционных заболеваний. В заключении обобщены преимущества и недостатки РПА по сравнению с другими методами амплификации.

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