Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-7613Central Research Institute for Epidemiology14310.36233/0372-9311-2017-2-60-65ORIGINAL RESEARCHESОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯANTIMICROBIAL AND ANTIFUNGAL ACTIVITY OF LICHENS PREVALENT IN BELARUSАНТИМИКРОБНАЯ И ПРОТИВОГРИБКОВАЯ АКТИВНОСТЬ ЭКСТРАКТОВ ЛИШАЙНИКОВ, РАСПРОСТРАНЕННЫХ НА ТЕРРИТОРИИ БЕЛАРУСИTapalskyD. V.ТапальскийД. В.
Russian Federation
PetrenevD. R.ПетреневД. Р.
Russian Federation
KhramchenkovaO. M.ХрамченковаО. М.
Russian Federation
DoroshkevichA. S.ДорошкевичА. С.
Russian Federation
Gomel State Medical UniversityГомельский государственный медицинский университетInstitute of Radiobiology of the National Academy of Sciences of BelarusИнститут радиобиологии Национальной академии наук БеларусиGomel State UniversityГомельский государственный университет28042017942606510042019Copyright ©; 2017, Tapalsky D.V., Petrenev D.R., Khramchenkova O.M., Doroshkevich A.S.Copyright ©; 2017, Тапальский Д.В., Петренев Д.Р., Храмченкова О.М., Дорошкевич А.С.2017Tapalsky D.V., Petrenev D.R., Khramchenkova O.M., Doroshkevich A.S.Тапальский Д.В., Петренев Д.Р., Храмченкова О.М., Дорошкевич А.С.https://creativecommons.org/licenses/by/4.0https://microbiol.crie.ru/jour/article/view/143

Aim. Study spectrum and expressiveness of antibacterial and antifungal properties oflichen extracts. Materials and methods. Antimicrobial activity of acetone extracts from Hypogymnia physodes, Xanthoria parietina, Evernia prunastri, Ramalina pollinaria, Cladonia arbuscula lichens was determined by micro-dilution methods in broth for 4 - 500 mcg/ml concentrations against 13 strains from ATCC collection and 6 clinical isolates. Results. High antibacterial activity of H. physodes and C. arbuscular extracts against staphylococci and enterococci was detected (MIC 31 - 62 mcg/ml). Antimicrobial activity against enterobacteria and Pseudomonas aeruginosa was absent for all the extracts. E.prunastri, H.physodes and C arbuscula extracts were active against Stenotrophomonas maltophilia strains (MIC 250 - 500 mcg/ml). Antifungal activity (MIC 500 mcg/ml for 4 Candida strains) was only detected for the E. prunastri extract. Conclusion. H.physodes and C. arbuscula lichens can be examined as a perspective source of antibacterial substances, effective against antibiotics resistant staphylococci, streptococci and S. maltophilia strains.

Цель. Изучение спектра и выраженности антибактериальных и противогрибковых свойств экстрактов лишайников. Материалы и методы. Антимикробную активность ацетоновых экстрактов из лишайников Hypogymnia physodes, Xanthoria parietina, Evernia prunastri, Ramalina pollinaria, Cladonia arbuscula определяли методом микроразведений в бульоне в диапазоне концентраций от 4 до 500 мкг/мл в отношении 13 штаммов из коллекции АТСС и 6 клинических изолятов. Результаты. Выявлена высокая антибактериальная активность экстрактов H.physodes и C.arbuscula в отношении стафилококов и энтерококков (МПК 31 - 62 мкг/мл). Антимикробная активность в отношении энтеробактерий и Pseudomonas aeruginosa отсутствовала у всех экс трактов. Экстракты E.prunastri, H.physodes и C.arbuscula были активны в отношении штаммов Stenotrophomonas maltophilia (МПК250 - 500 мкг/мл). Противогрибковая активность (МПК 500 мкг/мл для 4 штаммов Candida) выявлена только для экстракта E.prunastri. Заключение. Лишайники H.physodes и C.arbuscula можно рассматривать в качестве перспективных источников антибактериальных веществ, эффективных против антибиотикорезистентных штаммов стафилококков, стрептококков S.maltophilia.

Stenotrophomonas maltophilialichensextractsantibacterial activity. Stenotrophomonas maltophiliaлишайникиэкстрактыантибактериальная активностьBasile A., Rigano D., Loppi S. et al. Antiproliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin. Int. J. Mol. Sci. 2015, 16 (4): 7861-7875.Boustie J., Grube M. Lichens - a promising source of bioactive secondary metabolites. Plant. Genet. Resour. 2005, 3: 273-278.Chang Y.T., Lin C.Y., Chen Y.H., Hsueh P.R. Update on infections caused by Stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. Front. Microbiol. 2015, 6: 893.Grujicic D., Stosic I., Kosanic M. et al. Evaluation of in vitro antioxidant, antimicrobial, genotoxic and anticancer activities of lichen Cetraria islandica. Cytotechnology. 2014, 66 (5): 803-813.Gulluce M., Aslan A., Sokmen M. et al. Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana. Phytomedicine. 2006, 13 (7): 515521....Kosanic M., Manojlovic N., Jankovic S. et al. Evernia prunastri and Pseudoevernia fur-furaceae lichens and their major metabolites as antioxidant, antimicrobial and anticancer agents. Food. Chem. Toxicol. 2013, 53:112-118.Kosanic M., Rankovic B., Stanojkovic T et al. Biological activities and chemical composition of lichens from Serbia. EXCLI J. 2014, 13:1226-1238.Mitrovic T, Stamenkovic S., Cvetkovic V. et al. Platismatia glaucia and Pseudevernia furfuracea lichens as sources of antioxidant, antimicrobial and antibiofilm agents. EXCLI J. 2014, 13: 938-953.Oksanen I. Ecological and biotechnological aspects oflichens. Appl. Microbiol. Biotechnol. 2006, 73 (4): 723-734...Rankovic B., Misic M., Sukdolak S. Antimicrobial activity of extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla. Br. J. Biomed. Sci. 2007, 64 (4): 143-148.Studzinska-Sroka E., Holderna-Kedzia E., Galanty A. et al. In vitro antimicrobial activity of extracts and compounds isolated from Cladonia uncialis. Nat. Prod. Res. 2015; 29 (24): 2302-2307.Basile A., Rigano D., Loppi S. et al. Antiproliferative, antibacterial and antifungal activity of the lichen Xanthoria parietina and its secondary metabolite parietin. Int. J. Mol. Sci. 2015, 16 (4): 7861-7875.Boustie J., Grube M. Lichens - a promising source of bioactive secondary metabolites. Plant. Genet. Resour. 2005, 3: 273-278.Chang Y.T., Lin C.Y., Chen Y.H., Hsueh P.R. Update on infections caused by Stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. Front. Microbiol. 2015, 6: 893.Grujicic D., Stosic I., Kosanic M. et al. Evaluation of in vitro antioxidant, antimicrobial, genotoxic and anticancer activities of lichen Cetraria islandica. Cytotechnology. 2014, 66 (5): 803-813.Gulluce M., Aslan A., Sokmen M. et al. Screening the antioxidant and antimicrobial properties of the lichens Parmelia saxatilis, Platismatia glauca, Ramalina pollinaria, Ramalina polymorpha and Umbilicaria nylanderiana. Phytomedicine. 2006, 13 (7): 515521.Kosanic M., Manojlovic N., Jankovic S. et al. Evernia prunastri and Pseudoevernia fur-furaceae lichens and their major metabolites as antioxidant, antimicrobial and anticancer agents. Food. Chem. Toxicol. 2013, 53:112-118.Kosanic M., Rankovic B., Stanojkovic T et al. Biological activities and chemical composition of lichens from Serbia. EXCLI J. 2014, 13:1226-1238.Mitrovic T, Stamenkovic S., Cvetkovic V. et al. Platismatia glaucia and Pseudevernia furfuracea lichens as sources of antioxidant, antimicrobial and antibiofilm agents. EXCLI J. 2014, 13: 938-953.Oksanen I. Ecological and biotechnological aspects oflichens. Appl. Microbiol. Biotechnol. 2006, 73 (4): 723-734.Rankovic B., Misic M., Sukdolak S. Antimicrobial activity of extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla. Br. J. Biomed. Sci. 2007, 64 (4): 143-148.Studzinska-Sroka E., Holderna-Kedzia E., Galanty A. et al. In vitro antimicrobial activity of extracts and compounds isolated from Cladonia uncialis. Nat. Prod. Res. 2015; 29 (24): 2302-2307.