Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-7613Central Research Institute for Epidemiology13379ArticlesСтатьиReview ArticleUSING REVERSE GENETICS METHOD FOR DEVELOPING RECOMBINANT STRAINS OF INFLUENZA VIRUSES ACCEPTABLE FOR USE AS LIVE ATTENUATED VACCINESИСПОЛЬЗОВАНИЕ МЕТОДОВ ОБРАТНОЙ ГЕНЕТИКИ ДЛЯ ПОЛУЧЕНИЯ РЕКОМБИНАНТНЫХ ШТАММОВ ВИРУСА ГРИППА, ПРИГОДНЫХ В КАЧЕСТВЕ ЖИВЫХ АТТЕНУИРОВАННЫХ ВАКЦИНMerkulovV. AМеркуловВ. АLebedevV. NЛебедевВ. НPlekhanovaT. MПлехановаТ. МMaksimovV. AМаксимовВ. АKorovkinS. AКоровкинС. АMironovA. NМироновА. НBranch 48 of Central Research Institute of Ministry of Defense of Russian Federation — Virological Center, Sergiev PosadФилиал 48 Центрального НИИ Минобороны России — Вирусологический центр, Сергиев ПосадScientific-Manufacturing Organization «Microgen», Moscow, RussiaНПО «Микроген», Москва15042009862NO2 (2009)№2 (2009)11111709062023Copyright ©; 2009, Merkulov V.A., Lebedev V.N., Plekhanova T.M., Maksimov V.A., Korovkin S.A., Mironov A.N.Copyright ©; 2009, Меркулов В.А., Лебедев В.Н., Плеханова Т.М., Максимов В.А., Коровкин С.А., Миронов А.Н.2009Merkulov V.A., Lebedev V.N., Plekhanova T.M., Maksimov V.A., Korovkin S.A., Mironov A.N.Меркулов В.А., Лебедев В.Н., Плеханова Т.М., Максимов В.А., Коровкин С.А., Миронов А.Н.https://creativecommons.org/licenses/by/4.0https://microbiol.crie.ru/jour/article/view/13379

Perspectives of using reverse genetics methods for constructing of recombinant influenza virus strains acceptable for use as live attenuated vaccines are discussed. Using of attenuated NSvectors of influenza virus opens possibilities for the development of recombinant vaccines with optimal ratio of immunogenicity and safety. Reverse genetics is applicable for development of effective vaccines against new pathogens such as highly pathogenic avian influenza A/H5N1.

Обсуждаются перспективы использования методов обратной генетики для получения рекомбинантных штаммов вируса гриппа, пригодных для использования в качестве живых aттенуированных вакцин. Использование аттенуированных NS-векторов вируса гриппа открывает возможности для получения рекомбинантных вакцин с оптимальным соотношением иммуногенности и безопасности. Обратная генетика пригодна для создания эффективных вакцин против новых патогенов, таких как высокопатогенный вирус птичьего гриппа А/H5N1.

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