Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology131410.36233/0372-9311-266Research ArticleThe first case of detection of Listeria monocytogenes sequence types ST7, ST20, ST425 in wastewater during an investigation of water bodies in the Vologda regionAlekseevaElena A.<p>Cand. Sci. (Med.), Head, Bacteriological laboratory</p>kolombet@obolensk.orghttps://orcid.org/0000-0002-1860-0026PolosenkoOlga V.<p>Cand. Sci. (Biol.), leading researcher, Microbiological research department</p>kolombet@obolensk.orghttps://orcid.org/0000-0001-5961-9041FursovaNadezhda K.<p>Cand. Sci. (Biol.), leading researcher, Antimicrobial agents laboratory, Molecular microbiology department</p>kolombet@obolensk.orghttps://orcid.org/0000-0001-6053-2621AstashkinEvgeny I.<p>Cand. Sci. (Med.), senior researcher, Antimicrobial agents laboratory, Molecular microbiology department</p>kolombet@obolensk.orghttps://orcid.org/0000-0002-3559-9071BorzenkovValery N.<p>Cand. Sci. (Biol.), senior researcher, Antimicrobial agents laboratory, Molecular microbiology department</p>kolombet@obolensk.orghttps://orcid.org/0000-0002-6382-4299KislichkinaAngelina A.<p>Cand. Sci. (Biol.), senior researcher, Microbial collection department</p>kolombet@obolensk.orghttps://orcid.org/0000-0001-8389-2494KolombetLiubov V.<p>D. Sci. (Biol.), Head, Science Department</p>kolombet@obolensk.orghttps://orcid.org/0000-0001-9637-7790ShepelinAnatoly P.<p>D. Sci. (Biol.), Deputy director, State Research</p>kolombet@obolensk.orghttps://orcid.org/0000-0002-8253-7527MironovAndrey Yu.<p>D. Sci. (Med.), Professor, Head, Department of microbiology</p>kolombet@obolensk.orghttps://orcid.org/0000-0002-8544-5230Center of Hygiene and Epidemiology in the Vologda regionState Research Center for Applied Microbiology and BiotechnologyG.N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology270920229944534642709202227092022Copyright © 2022, Alekseeva E.A., Polosenko O.V., Fursova N.K., Astashkin E.I., Borzenkov V.N., Kislichkina A.A., Kolombet L.V., Shepelin A.P., Mironov A.Y.2022<p><strong>Introduction</strong>. Listeria monocytogenes is an important human pathogen causing various forms of listeriosis, including foodborne infections, meningitis, neonatal sepsis, and abortion. Listeria are common all over the world.</p>
<p><strong>The purpose</strong> of the study was to conduct microbiological monitoring of L. monocytogenes in water reservoirs near livestock premises in the Vologda district of the Vologda region.</p>
<p><strong>Materials and methods</strong>. Bacterial cultures were isolated using two methods, titration and filtration, followed by analysis using methods of conventional bacteriology, serotyping, and species identification by instrumental procedures such as whole genome sequencing, and bioinformatic analysis.</p>
<p><strong>Results</strong>. Three isolates of L. monocytogenes and one isolate of Listeria innocua were isolated from 12 analyzed water samples (wastewater — 6, river water — 4, and storm water — 2 samples). whole genome sequencing of three L. monocytogenes strains attributed them to the evolutionary line II, and to three sequence types and two serogroups ST425(1/2a-3a), ST20(1/2a-3a), ST7 (4a-4c). The strains are shown to belong to multiple drug resistant ones conferring resistance to three functional groups of antibacterials such as tetracyclines, macrolides, and sulfonamides. Antibiotic resistance genes (fox, psp-like, lin,norB,sul), virulence Islands LIPI-1 and LIPI-2, and virulence genes inlABCJ, oatA, ami, gtcA, vip, and lisK in genomes of the strain were identified. Stress tolerance Island SSI-1 was identified in one strain.</p>
<p><strong>Conclusions.</strong> The data obtained indicate contamination of water sources near the livestock premises with L. monocytogenes strains possessing high pathogenic potentiality for outbreaks of listeriosis in humans. This shows the necessity of careful monitoring of water sources for the presence of the causative agent of listeriosis as well as the implementing of anti-epidemic measures.</p>Listeria monocytogenesmonitoringgenetic linessequence typesvirulence genesstress tolerance genesListeria monocytogenesмониторинггенетические линиисиквенс-типыгены вирулентностигены стрессоустойчивости[Centers for Disease Control and Prevention (CDC). Vital signs: Listeria illnesses, deaths, and outbreaks - United States, 2009- 2011. MMWR Morb Mortal Wkly Rep. 2013; 62(22): 448-52.][European Food Safety Authority (EFSA). EU summary report on trends and sources of zoonoses, zoonotic agents and foodborne outbreaks in 2015. 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