Journal of microbiology, epidemiology and immunobiologyJournal of microbiology, epidemiology and immunobiology0372-93112686-7613Central Research Institute for Epidemiology126210.36233/0372-9311-231UnknownWhole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen regionKataevaL. V.<p>Lyubov V. Kataeva — D. Sci. (Med.), leading researcher, Head, Bacteriological laboratory.</p><p>Tyumen</p>info@tniikip.rospotrebnadzor.ruhttps://orcid.org/0000-0001-9966-8454KolotovaO. N.<p>Olga N. Kolotova — junior researcher, Bacteriological laboratory.</p><p>Tyumen</p>fake@neicon.ruhttps://orcid.org/0000-0002-0798-5549StepanovaT. F.<p>Tatyana F. Stepanova — D. Sci. (Med.), Professor, Director.</p><p>Tyumen</p>fake@neicon.ruhttps://orcid.org/0000-0002-6289-6274KislichkinaA. A.<p>Angelina A. Kislichkina — Cand. Sci. (Biol.), senior researcher, Department of collection cultures.</p><p>Obolensk</p>fake@neicon.ruhttps://orcid.org/0000-0001-8389-2494ShishkinaL. A.<p>Lidia A. Shishkina — Cand. Sci. (Biol.), junior researcher, Department of collection cultures.</p><p>Obolensk</p>fake@neicon.ruhttps://orcid.org/0000-0001-8615-1907MukhinaT. N.<p>Tatyana N. Mukhina — Cand. Sci. (Biol.), senior researcher, Department of collection cultures.</p><p>Obolensk</p>fake@neicon.ruhttps://orcid.org/0000-0001-5829-0512Tyumen Region Infection Pathology Research InstituteState Research Center for Applied Microbiology and Biotechnology280720229933433522807202228072022Copyright © 2022, Kataeva L.V., Kolotova O.N., Stepanova T.F., Kislichkina A.A., Shishkina L.A., Mukhina T.N.2022<p><strong>Introduction.</strong> is to The analysis of the genetic relatedness of isolates aiming to find the source of infection is an important task of nosocomial infection control. The most common causative agent of healthcare-associated infections is Acinetobacter baumannii.</p>
<p><strong>Objective.</strong> To evaluate the results of whole genome sequencing of A. baumannii bacteria isolated from clinical samples of patients undergoing inpatient treatment in the northern territories of the Tyumen region.</p>
<p><strong>Materials and methods</strong>. Nine isolates of A. baumannii from the clinical material of patients were studied. Bacterial cultures were identified by mass spectrometry. Whole genome sequencing, multilocus sequence typing and search for markers of antibiotic resistance were performed.</p>
<p><strong>Results.</strong> The studied strains belonged to sequence types ST2 and ST187, and to the international clonal complex CC2. All A. baumannii isolates were found to have beta-lactamase genes, as well as genes for resistance to aminoglycosides, to the MLS group of antibiotics, and to tetracyclines. The presence of a cluster of genes associated with virulence was detected: those responsible for the synthesis of acinetobactin and iron binding, surface antigen 1 and porin.</p>
<p><strong>Conclusion.</strong> Based on data of a single nucleotide polymorphism (SNP) analysis, A. baumannii isolates from the clinical material of patients of healthcare institution #1 belong mainly to one bacterial strain. Isolates of A. baumannii from the clinical material of patients of healthcare institution #2 are closely related. The ability to distinguish clinical isolates of A. baumannii at the level of several SNPs per genome will improve the identification of the source of infection, and whole genome sequencing data can contribute to the rational prescription of antibiotic therapy and the correction of disinfection and antiseptic measures.</p>Acinetobacter baumanniiclinical strainswhole genome sequencingresistance genessequence typesAcinetobacter baumanniiклинические изолятыполногеномное секвенированиегены резистентностисиквенс-типы[1. Янович Ю.А., Рачина С.А., Сухорукова М.В., Савочкина Ю.А., Вацик М.В., Петров А.А. 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