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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Journal of microbiology, epidemiology and immunobiology</journal-id><journal-title-group><journal-title xml:lang="en">Journal of microbiology, epidemiology and immunobiology</journal-title><trans-title-group xml:lang="ru"><trans-title>Журнал микробиологии, эпидемиологии и иммунобиологии</trans-title></trans-title-group></journal-title-group><issn publication-format="print">0372-9311</issn><issn publication-format="electronic">2686-7613</issn><publisher><publisher-name xml:lang="en">Central Research Institute for Epidemiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">1196</article-id><article-id pub-id-type="doi">10.36233/0372-9311-262</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>ORIGINAL RESEARCHES</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Еffect of the essential oil of <italic>Satureja montana</italic> L. on the growth of cultures of conditionally pathogenic microorganisms</article-title><trans-title-group xml:lang="ru"><trans-title>Влияние эфирного масла чабера горного на рост культур условно-патогенных микроорганизмов</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2113-4107</contrib-id><name-alternatives><name xml:lang="en"><surname>Postnikova</surname><given-names>Olga N.</given-names></name><name xml:lang="ru"><surname>Постникова</surname><given-names>Ольга Николаевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>senior lecturer, Department of microbiology, virology and immunology</p></bio><bio xml:lang="ru"><p>старший преподаватель каф. микробиологии, вирусологии и иммунологии</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1166-4585</contrib-id><name-alternatives><name xml:lang="en"><surname>Shevkoplyas</surname><given-names>Ludmila A.</given-names></name><name xml:lang="ru"><surname>Шевкопляс</surname><given-names>Людмила Александровна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>assistant, Department of microbiology, virology and immunology</p></bio><bio xml:lang="ru"><p>ассистент каф. микробиологии, вирусологии и иммунологии</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0055-8605</contrib-id><name-alternatives><name xml:lang="en"><surname>Kuevda</surname><given-names>Tatyana A.</given-names></name><name xml:lang="ru"><surname>Куевда</surname><given-names>Татьяна Алексеевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>junior researcher, Department of field crop</p></bio><bio xml:lang="ru"><p>м.н.с. отд. полевых культур</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff2"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6451-7285</contrib-id><name-alternatives><name xml:lang="en"><surname>Sataieva</surname><given-names>Tatiana P.</given-names></name><name xml:lang="ru"><surname>Сатаева</surname><given-names>Татьяна Павловна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>D. Sci. (Med.), Head, Department of microbiology, virology and immunology</p></bio><bio xml:lang="ru"><p>д.м.н., зав. каф. микробиологии, вирусологии и иммунологии</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Kirsanova</surname><given-names>Marina A.</given-names></name><name xml:lang="ru"><surname>Кирсанова</surname><given-names>Марина Александровна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Cand. Sci. (Biol.), Associate Professor, Department of microbiology, virology and immunology</p></bio><bio xml:lang="ru"><p>к.б.н., доц. каф. микробиологии, вирусологии и иммунологии</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2470-5374</contrib-id><name-alternatives><name xml:lang="en"><surname>Logadyr</surname><given-names>Tatyana A.</given-names></name><name xml:lang="ru"><surname>Логадырь</surname><given-names>Татьяна Алексеевна</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><bio xml:lang="en"><p>Cand. Sci. (Med.), Associate Professor, Department of microbiology, virology and immunology</p></bio><bio xml:lang="ru"><p>к.м.н., доц. каф. микробиологии, вирусологии и иммунологии</p></bio><email>tanzcool@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">S.I. Georgievsky Medical Academy, V.I. Vernadsky Crimean Federal University</institution></aff><aff><institution xml:lang="ru">Медицинская академия имени С.И. Георгиевского Крымского федерального университета имени В.И. Вернадского</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">Research Institute of Agriculture of Crimea</institution></aff><aff><institution xml:lang="ru">Научно-исследовательский институт сельского хозяйства Крыма</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2022-12-15" publication-format="electronic"><day>15</day><month>12</month><year>2022</year></pub-date><volume>99</volume><issue>6</issue><issue-title xml:lang="en"/><issue-title xml:lang="ru"/><fpage>701</fpage><lpage>707</lpage><history><date date-type="received" iso-8601-date="2022-03-24"><day>24</day><month>03</month><year>2022</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2022, Postnikova O.N., Shevkoplyas L.A., Kuevda T.A., Sataieva T.P., Kirsanova M.A., Logadyr T.A.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2022, Постникова О.Н., Шевкопляс Л.А., Куевда Т.А., Сатаева Т.П., Кирсанова М.А., Логадырь Т.А.</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="en">Postnikova O.N., Shevkoplyas L.A., Kuevda T.A., Sataieva T.P., Kirsanova M.A., Logadyr T.A.</copyright-holder><copyright-holder xml:lang="ru">Постникова О.Н., Шевкопляс Л.А., Куевда Т.А., Сатаева Т.П., Кирсанова М.А., Логадырь Т.А.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://microbiol.crie.ru/jour/article/view/1196">https://microbiol.crie.ru/jour/article/view/1196</self-uri><abstract xml:lang="en"><p><bold>Introduction. </bold>Essential oils contain antimicrobial components that are highly active against a wide range of microorganisms. Essential oils are natural, environmentally safe, low-toxic substances with a minimal list of side effects; no antimicrobial resistance is formed to them.</p> <p><bold>The aim</bold> of the research was to study the influence of the essential oil of <italic>Satureja montana </italic>L., growing in the Crimea, on the growth of cultures of opportunistic microorganisms.</p> <p><bold>Materials and methods. </bold>The short-term effect of savory oil on the growth of referenсе strains of microorganisms was studied in accordance with the European Standard for determining the rate of inactivation of microorganisms by the test substance (1997). To study the long-term effect of savory oil on clinical isolates of <italic>Staphylococcus aureus</italic>, we used the method of dilutions in a liquid medium, followed by measurement of the optical density of growth of the suspension culture biomass. The effect of savory oil on the formation of biofilms by clinical isolates of<italic> S. aureus </italic>was also studied.</p> <p><bold>Results.</bold> Whole savory oil and its dilutions of 1 : 10 and 1 : 100 with short-term action (10–60 min) completely suppressed the growth of referenсе strains of bacteria; growth of the referenсе strain <italic>Candida albicans</italic> CCM 885 was inhibited only by whole oil and a 1 : 10 dilution, while a 1 : 100 dilution had a bacteriostatic effect. Dilutions of essential oil 1 : 100 and 1 : 1000 had a pronounced antibacterial effect on the suspension culture of clinical isolates of <italic>S. aureus</italic>. Savory oil also inhibited biofilm formation by 11 isolates <italic>S. aureus</italic>.</p> <p><bold>Conclusion.</bold> The essential oil of <italic>Satureja montana</italic> L. exhibits a pronounced antimicrobial effect against referenсе strains of <italic>S. aureus</italic> ATCC 25923, <italic>Escherichia coli </italic>ATCC 25922 and fungi <italic>C. albicans</italic> CCM 885. The antibacterial effect of this essential oil on clinical isolates of S. aureus allows us to offer it as a component of combined preparations for the treatment of infections caused by antibiotic-resistant strains of staphylococcus.</p></abstract><trans-abstract xml:lang="ru"><p><bold>Введение.</bold> Эфирные масла содержат противомикробные компоненты, которые высокоактивны против широкого спектра микроорганизмов. Эфирные масла являются натуральными, экологически безопасными, низкотоксичными веществами, к ним не формируется резистентность микроорганизмов, они обладают минимальным перечнем побочных эффектов.</p> <p><bold>Целью </bold>исследований было изучение влияния эфирного масла чабера горного (<italic>Satureja montana </italic>L.) — МЧГ, растущего в Крыму, на рост культур условно-патогенных микроорганизмов.</p> <p><bold>Материалы и методы.</bold> Кратковременное действие МЧГ на рост референтных штаммов микроорганизмов исследовали в соответствии с Европейским стандартом определения скорости инактивации микроорганизмов исследуемым веществом (1997). Для изучения длительного воздействия МЧГ на клинические изоляты <italic>Staphylococcus aureus </italic>использовали метод разведений в жидкой среде с последующим измерением оптической плотности нарастания биомассы суспензионной культуры. Исследовали также влияние МЧГ на образование биоплёнок клиническими изолятами <italic>S. аureus</italic>.</p> <p><bold>Результаты.</bold> Цельное МЧГ и его разведения 1 : 10 и 1 : 100 при кратковременном действии (10–60 мин) полностью подавляли рост референтных штаммов условно-патогенных бактерий; рост референтного штамма <italic>Candida аlbicans</italic> ССМ 885 ингибировался только цельным МЧГ и разведением 1 : 10, а разведение МЧГ 1 : 100 оказывало бактериостатический эффект. Разведения МЧГ 1 : 100 и 1 : 1000 оказывали выраженное антибактериальное действие на суспензионную культуру клинических изолятов <italic>S. aureus</italic>. МЧГ подавляло образование биоплёнок 11 изолятами <italic>S. aureus</italic>.</p> <p><bold>Заключение.</bold> МЧГ проявляет выраженное антимикробное действие в отношении референтных штаммов <italic>S. aureus</italic> АТСС 25923, <italic>Escherichia coli</italic> АТСС 25922 и грибов <italic>C. albicans</italic> ССМ 885. Антибактериальное действие МЧГ на клинические изоляты <italic>S. aureus</italic> позволяет предлагать его в качестве компонента комбинированных препаратов для лечения инфекций, вызванных антибиотикоустойчивыми штаммами стафилококка.</p></trans-abstract><kwd-group xml:lang="en"><kwd>essential oil of Satureja montana L.</kwd><kwd>Staphylococcus aureus</kwd><kwd>Escherichia coli</kwd><kwd>Candida albicans</kwd><kwd>biofilms</kwd><kwd>antimicrobial activity</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>эфирное масло чабера</kwd><kwd>Staphylococcus aureus</kwd><kwd>Escherichia coli</kwd><kwd>Candida аlbicans</kwd><kwd>биоплёнки</kwd><kwd>антимикробное действие</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution xml:lang="ru">Министерство науки и высшего образования Российской Федерации</institution></institution-wrap><institution-wrap><institution xml:lang="en">Ministry and Higher Education of the Russian Federation</institution></institution-wrap></funding-source><award-id>075-15-2021-1323</award-id></award-group></funding-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><citation-alternatives><mixed-citation xml:lang="en">Egorov A.M., Sazykin Yu.O. 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